Restriction Enzymes
New England Biolabs, Inc. HindIII – 10000 units
A restriction endonuclease that recognizes the sequence A^AGCT_T.
New England Biolabs, Inc. AlwNI – 2500 units
A restriction endonuclease that recognizes the sequence CAG_NNN^CTG.
New England Biolabs, Inc. EarI – 500 units
A restriction endonuclease that recognizes the sequence CTCTTCN^NNN_.
New England Biolabs, Inc. BfaI – 500 units
A restriction endonuclease that recognizes the sequence C^TA_G.
New England Biolabs, Inc. SbfI-HF – 500 units
A high fidelity restriction endonuclease that recognizes the sequence CC_TGCA^GG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. BglII – 2000 units
A restriction endonuclease that recognizes the sequence A^GATC_T.
New England Biolabs, Inc. BspMI – 100 units
A restriction endonuclease that recognizes the sequence ACCTGCNNNN^NNNN_.
New England Biolabs, Inc. EcoNI – 1000 units
A restriction endonuclease that recognizes the sequence CCTNN^N_NNAGG.
New England Biolabs, Inc. PmlI – 2000 units
A restriction endonuclease that recognizes the sequence CAC_^GTG.
New England Biolabs, Inc. BspHI – 500 units
A restriction endonuclease that recognizes the sequence T^CATG_A.
New England Biolabs, Inc. AseI – 2000 units
A restriction endonuclease that recognizes the sequence AT^TA_AT.
New England Biolabs, Inc. AflII – 10000 units
A restriction endonuclease that recognizes the sequence C^TTAA_G.
New England Biolabs, Inc. BsrDI – 200 units
A restriction endonuclease that recognizes the sequence GCAATG_NN^.
New England Biolabs, Inc. BtgZI – 500 units
A restriction endonuclease that recognizes the sequence GCGATGNNNNNNNNNN^NNNN_.
New England Biolabs, Inc. BsaHI – 2000 units
A restriction endonuclease that recognizes the sequence GR^CG_YC.