Restriction Enzymes
American Research Products Inc General SPH ELISA Kit
This General Sphingosine (SPH) ELISA Kit is a precoated Competitive Inhibition ELISA assay designed to detect and quantify the target analyte in tissue homogenates cell lysates and other biological fluids This product is for Research Use Only
New England Biolabs, Inc. PstI – 10000 units
A restriction endonuclease that recognizes the sequence C_TGCA^G.
New England Biolabs, Inc. MslI – 500 units
A restriction endonuclease that recognizes the sequence CAYNN_^NNRTG.
Sigma Aldrich Fine Chemicals Biosciences β-Glucuronidase from Escherichia coli | CAS 9027-70-9 | 1,000,000 Units
β-glucuronidase from E. coli | Purity: ≥90% | Mol Wt: 68 kDa | CAS No: 9027-70-9 | Unit: 1,000,000 Units
New England Biolabs, Inc. Exonuclease V (RecBCD) – 5000 units
Exonuclease V, a RecBCD complex from E. coli has several different enzyme activities, including an ATP-dependent single-stranded DNA endonuclease activity, ss- and ds- DNA exonuclease activity. The hydrolysis in each case is bi-directional (from both the 3' and 5' ends) and processive, producing oligonucleotides. All Exonuclease V activities have divalent cation requirements. Mg2+ is required for the exonuclease activity, while Ca2+ inhibits the exonuclease activity and allows double-stranded DNA unwinding (helicase activity) without hydrolysis.
New England Biolabs, Inc. EciI – 100 units
A restriction endonuclease that recognizes the sequence GGCGGANNNNNNNNN_NN^.
New England Biolabs, Inc. PpuMI – 2500 units
A restriction endonuclease that recognizes the sequence RG^GWC_CY.
New England Biolabs, Inc. CspCI – 500 units
A restriction endonuclease that recognizes the sequence _NN^NNNNNNNNNNNCAANNNNNGTGGNNNNNNNNNN_NN^.
New England Biolabs, Inc. FatI – 250 units
A restriction endonuclease that recognizes the sequence ^CATG_.
New England Biolabs, Inc. HpyAV – 100 units
A restriction endonuclease that recognizes the sequence CCTTCNNNNN_N^.
New England Biolabs, Inc. PciI – 1000 units
A restriction endonuclease that recognizes the sequence A^CATG_T.
New England Biolabs, Inc. T7 Endonuclease I – 250 units
T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5' to the mismatch. The protein is the product of T7 gene 3.
New England Biolabs, Inc. Endonuclease V – 250 units
Endonuclease V is a repair enzyme found in E. coli that recognizes deoxyinosine, a deamination product of deoxyadenosine in DNA. Endonuclease V, often called deoxyinosine 3 endonuclease, recognizes DNA containing deoxyinosines (paired or not) on double-stranded DNA, single-stranded DNA with deoxyinosines and to a lesser degree, DNA containing abasic sites (ap) or urea, base mismatches, insertion/deletion mismatches, hairpin or unpaired loops, flaps and pseudo-Y structures. It is believed that Endonuclease V needs another protein to repair the DNA, as it does not remove the deoxyinosine or the damaged bases. Endonuclease V cleaves the second phosphodiester bonds 3' to the mismatch of deoxyinosine, leaving a nick with 3'-hydroxyl and 5'-phosphate.
New England Biolabs, Inc. Terminal Transferase – 500 units
Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. Protruding, recessed or blunt-ended double or single-stranded DNA molecules serve as a substrate for TdT. The 58.3 kDa enzyme does not have 5' or 3' exonuclease activity. The addition of Co2+ in the reacton makes tailing more efficient.